This post is a repost of a North Carolina State University press release: In Ironic Twist, CRISPR System Used to Befuddle Bacteria Image courtesy of Rodolphe Barrangou, rbarran@ncsu.edu North Carolina State University Base editing within a community context. (A) An EcoFAB device filled with quartz sand (Left) and an EcoFAB device loaded with LB and synthetic soil community (Right). (B) EcoFAB community editing strategy overview. (C) Blue or white E. coli colonies resulting from synthetic … [Read more...]
Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing
Scientists from the Innovative Genomics Institute at UC Berkeley, North Carolina State University and Lawrence Berkeley National Lab use RNA-targeting CRISPR systems to drive genome editing across phage diversity. Image courtesy of Davian Ho: CRISPR-Cas13 creates an evolutionary bottleneck by targeting the RNA of diverse phages, allowing for edited phages (orange) to be specifically enriched. Bacterial viruses (bacteriophages or phages) are estimated to be among the most diverse and … [Read more...]
Editing microbial genomes and communities in the laboratory
Species- and Site-Specific Genome Editing in Complex Bacterial Communities A novel toolset and approach to modify bacterial genomes within complex laboratory consortia. Rubin, BE; Diamond, S; Cress, BF; Crits-Christoph A, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. (2021) Targeted Genome Editing of Bacteria Within Microbial Communities. Nature Microbiology 2021 [DOI]: … [Read more...]
