m-CAFEs researchers present new database for the collection, classification and utilization of CRISPR-Cas enzymes. The Science m-CAFEs researchers developed CasPEDIA, the Cas Protein Effector Database of Information and Assessment, a new database which provides summary information about the capabilities and limitations of Class 2 Cas technologies to facilitate tool selection and highlight opportunities for future biotechnological development. They also introduce CasID, a Cas enzyme … [Read more...]
Genome-wide fitness profiling reveals molecular mechanisms that bacteria use to interact with Trichoderma atroviride exometabolites
The Science The rhizosphere that surrounds plant roots is a hotspot for bacteria, fungi, and other microorganisms. While how roots interact with bacteria is relatively understood, the dynamics between bacteria and fungi in the rhizosphere are less clear. In this work, m-CAFEs researchers at UC Berkeley focused on Trichoderma atroviride, a fungus in the root zone that produces complex substances influencing plant-microbe interactions. Observing lab-altered bacteria revealed that exposure to … [Read more...]
Researchers Close Rhizosphere Microbiome Knowledge Gaps
The Science m-CAFEs researchers studied microbial communities at different zones of the roots in three different types of growth containers: conventional pots, tubes, and standardized fabricated ecosystems (EcoFABs), designed to mimic conditions of a natural environment. The goal was to characterize microbial communities on the tips of the roots, the soil at the base of the roots and regular bulk soil that was not in the rhizosphere environment. The EcoFAB 2.0 is a standardized device for … [Read more...]
